Widefield and TIRF Microscopy

EPI and TIRF FacilitiesEpifluorescence (widefield) microscopy allows for fast acquisition of 3D or 4D data sets as an EMCCD camera is used and an entire field of view is captured at one time. TIRFM uses the unique properties of an evanescent wave to selectively excite fluorophores within a restricted region i.e. the plasma membrane of a cell.

ESRIC houses an epifluorescent Olympus IX81 fully motorised microscope (see specification) controlled by Olympus CellR software, which is equipped with total internal reflection fluorescence microscopy (TIRFM) capabilities. 

Live cell recordings can be aquired at high temporal resolution (10 ms frame rates). This system is capable of live cell imaging on a heated stage with optional CO2 perfusion. This system uses a coverslip chamber which fits 25 mm coverslips (SLS Cat No. MIC3350). In addition a stage for mounted slides is provided.

This system is available at the Heriot-Watt University site, for all enquires please contact Ali Dun This email address is being protected from spambots. You need JavaScript enabled to view it..


A Large Dense Core Vesicle tracked over time at the plasma membrane of a cell using Imaris (Bitplane) software available in ESRIC.

Confocal Laser Scanning Microscopy (CLSM)                                                                           

Confocal our facilitiesA reconstructed 3D stack of intestinal tissue from a mouse stained with two fibrous structural proteins in red and green

Confocal laser scanning microscopy (CLSM) is an optical imaging technique used to acquire highly detailed information of thin optical sections, a process known as optical sectioning. This is possible through the rejection of out of focus light using pinholes and the pixel by pixel illumination of the sample.

Techniques possible on this system include Fluorescence correlation spectroscopy (FCS), time correlated single photon counting - fluorescence lifetime imaging microscopy (TCSPC-FLIM) and gated-stimulated emission depletion microscopy (gSTED).

ESRIC houses a Leica SP5 SMD gSTED laser scanning confocal microscope (see specification) equiped with a super continuum white light laser. This system is capable of live cell imaging on a heated stage with optional CO2 perfusion. This system uses a coverslip chamber which fits 25 mm coverslips (SLS Cat No. MIC3350). In addition a stage for mounted slides is provided.

This microscope is available at the Heriot-Watt University site, contact Ali Dun This email address is being protected from spambots. You need JavaScript enabled to view it.