PALM

PALM intro 1Photoactivatable localisation microscopy (PALM) provides quantifiable localisation of single molecules with nanometer resolution, within ESRIC at the plasma membrane of cells.

PALM relies on the use of photoactivatable fluorescent proteins and the ability to irreversibly 'switch them off'. Serial photoactivation and photobleaching reduces the density of single molecules being imaged and allows their positions to be mapped.

PALM can be performed in TIRF on the epifluorescent Olympus IX81 fully motorised TIRFM microscope (see specification) or images can be acquired deeper inside the cell on the Nikon 3D-N-STORM system (see specification).

PALM exclusively in TIRF is available at the Heriot-Watt University site, for all enquires please contact Ali Dun This email address is being protected from spambots. You need JavaScript enabled to view it.. PALM with 3D information is available at the University of Edinburgh site, please contact Ann Wheeler This email address is being protected from spambots. You need JavaScript enabled to view it..

STORM                                                                                   

STORM Intro

The reversible deactivation of single fluorescent molecules can be applied to visualise and localise single molecules within a cellular structure. Ground-state depletion imaging microscopy (GSDIM) and Stochastic optical reconstruction microscopy (STORM) are two very similar techniquues that can be performed in ESRIC (termed STORM for simplicity).

STORM can be performed in TIRF on the epifluorescent Olympus IX81 fully motorised TIRFM microscope (see specification) or images can be acquired deeper inside the cell on the Nikon 3D-N-STORM system (see specification).

STORM exclusively in TIRF is available at the Heriot-Watt University site, for all enquires please contact Ali Dun This email address is being protected from spambots. You need JavaScript enabled to view it.. STORM with 3D information is available at the University of Edinburgh site, please contact Ann Wheeler This email address is being protected from spambots. You need JavaScript enabled to view it. for more information.

 

 

Atomic Force Microscopy

AFM Combined1

Atomic Force Microscopy is a technique which provides resolution on the atomic scale and can be used to create a quantitative 3D image of a cell surface as well as information regarding the mechanical, electrical and magnetic characteristics of your sample.

Combining a cantilever comprising of only a few atoms at its sharpest point and a laser system, a topographical map can be generated displaying the sample’s dimensional qualities. The cantilever is set to oscillate, or ‘’tap’’, to prevent prolonged contact with the sample minimising damage. As the sharp tip approaches the sample interatomic forces repel the tip and this allows for the topographical map to be plotted based on this repulsion.

ESRIC houses a JPK Nanowizard 3 Bioscience optimised for live cell imaging, mounted on a Zeiss Axio Observer D1 Microscope completely contained in an acoustic enclosure.

This microscope is available at the Heriot-Watt University site, for all enquiries please contact Ali Dun This email address is being protected from spambots. You need JavaScript enabled to view it.