Stimulated emission depletion (STED) microscopy is a super-resolution technique capable of up to 50nm resolution through the selective deactivation of fluorescent molecules using two synchronised lasers. Gated fluorescence detection-STED (g-STED) reduces the STED intensity in the sample and improves further the resolution.
Gated-STED is performed on a Lecia SP5 SMD g-STED microscope (see specification) equipped with a super continuum white light laser and a 592nm depletion laser.
A list of STED dyes can be found at this link, for the ESRIC g-STED any that are depleted by 592nm light are appropriate.
Nikon N-SIM Structured Illumination Microscopy (SIM) utilises a structured light pattern to image fluorophores within a biological specimen in 3D at double the conventional resolution. This technique can achieve up to 120 nm in xy and 300 nm in z (sample dependent) in 4 colours.
This super resolution technique is performed on a Nikon N-SIM system (see specification).
Image credit Alan Egan, ESRIC PhD Student