Gated-STED

2colourSTEDStimulated emission depletion (STED) microscopy is a super-resolution technique capable of up to 50nm resolution through the selective deactivation of fluorescent molecules using two synchronised lasers. Gated fluorescence detection-STED (g-STED) reduces the STED intensity in the sample and improves further the resolution.

Gated-STED is performed on a Lecia SP5 SMD g-STED microscope (see specification) equipped with a super continuum white light laser and a 592nm depletion laser. 

A list of STED dyes can be found at this link, for the ESRIC g-STED any that are depleted by 592nm light are appropriate.

This microscope is available at Heriot Watt University site, for all enquiries please contact Ali Dun This email address is being protected from spambots. You need JavaScript enabled to view it.. Image credit: Andrew Jarjour, SCRM, University of Edinburgh

SIM                                                                                

SIM scaledNikon N-SIM Structured Illumination Microscopy (SIM) utilises a structured light pattern to image fluorophores within a biological specimen in 3D at double the conventional resolution. This technique can achieve up to 120 nm in xy and 300 nm in z (sample dependent) in 4 colours.

This super resolution technique is performed on a Nikon N-SIM system (see specification).  

This N-SIM system microscope is available at the University of Edinburgh site, contact Ann Wheeler This email address is being protected from spambots. You need JavaScript enabled to view it..

Image credit Alan Egan, ESRIC PhD Student