Nikon N-STORM V

IGC, University of Edinburgh site

A 3D single molecule localisation microscopy system. Capable of PALM, STORM, PAINT, SRRF and other SMLM methods. Lateral localisation precision 10nm 

  • Lasers: 405, 488, 561, 647 
  • Objectives: 60x, 100x 1.4NA oil, 100x 1.35NA Silicone.
  • Suggested dyes: Alexa Fluor 647, Atto 647, JaneliaFluor 549, Alexa Fluor 568
  • Software: NIS-Elements 

Picture of STORM Microscope at IGC site

Nikon N-SIM/SORA 

IGC, University of Edinburgh site

A hybrid structured illumination system. Offering standard structured illumination microscopy (SIM) and instant SIM for live imaging allow lateral resolutions of 115nm and 120nm respectively

  • Lasers (nm): 405, 488, 561, 638 (SORA) 640 (SIM) 
  • Objectives: 100x 1.49 Oil, 100x 1.35 NA Silicone, 10x 0.3 
  • Suggested dyes: DAPI, Alexa 405, Alexa Fluor 488, Alexa Fluor 568, Alexa Fluor 647, SiRdayes
  • Software: NIS-Elements

Picture of SIM-SORA Microscope at !GC Wite

Leica SP8 3X Tau STED

IB3, Heriot-Watt University Site

A stimulated emission depletion (STED) 3D microscope, capable of 40 nm lateral and 100 nm axial resolutions.

  • Lasers: 405nm, White Light. Depetion lines 592, 775nm
  • Fluorophores: AlexaFluor594, Abberior STAR Red, Abberior STAR Green. 
  • Objectives: 10x, 40x ,63x Oil, 100x Oil
  • Software: LAS-X
  • Applications: See our our education centrefor more information

 

Leica STED workshop HW

Olympus Cell Xcellence

IB3, Heriot-Watt University Site

A high resolution total internal reflection fluorescence (TIRF) imaging system designed for live imaging application including single particle tracking-photoactivatable localisation microscope (spt-PALM)

  • Lasers, 488, 560, 640nm
  • Fluorophores: eGFPmClover2, mRFP, MKate 
  • Objectives: 10x, 40x ,63x Oil, 100x Oil
  • Software: Olympus Cell Sens, Thunderstorm (Image J)

 CelXplorer

Leica SP5 SMD Confocal

IB3, Heriot-Watt University Site

A point scanning confocal system set up for analysis of molecular dynamics. The system is equipped with with Picoquant APDs for Fluorescence correlation Spectroscopy (FCS), Forester resonance energy transfer (FRET) and Fluroescence Lifetime Imaging Microscopy (FLIM).

  • Lasers, 405nm White light super-continuum laser
  • Fluorophores: eGFPmCherry, mTeal, Venus 
  • Objectives: 10x, 40x ,63x Oil, 100x Oil
  • Software: LAS, Symphotime.