PALM

PALM intro 1Photoactivatable localisation microscopy (PALM) provides quantifiable localisation of single molecules with nanometer resolution, within ESRIC at the plasma membrane of cells.

PALM relies on the use of photoactivatable fluorescent proteins and the ability to irreversibly 'switch them off'. Serial photoactivation and photobleaching reduces the density of single molecules being imaged and allows their positions to be mapped.

PALM can be performed in TIRF on the epifluorescent Olympus IX81 fully motorised TIRFM microscope (see specification) or images can be acquired deeper inside the cell on the Nikon 3D-N-STORM system (see specification).

PALM exclusively in TIRF is available at the Heriot-Watt University site, for all enquires please contact This email address is being protected from spambots. You need JavaScript enabled to view it.. PALM with 3D information is available at the University of Edinburgh site, please contact Ann Wheeler This email address is being protected from spambots. You need JavaScript enabled to view it..

STORM                                                                                   

STORM Intro

 

The reversible deactivation of single fluorescent molecules can be applied to visualise and localise single molecules within a cellular structure. Ground-state depletion imaging microscopy (GSDIM) and Stochastic optical reconstruction microscopy (STORM) are two very similar techniquues that can be performed in ESRIC (termed STORM for simplicity).

STORM can be performed in TIRF on the epifluorescent Olympus IX81 fully motorised TIRFM microscope (see specification) or images can be acquired deeper inside the cell on the Nikon 3D-N-STORM system (see specification).

STORM exclusively in TIRF is available at the Heriot-Watt University site, for all enquires please contact This email address is being protected from spambots. You need JavaScript enabled to view it.. STORM with 3D information is available at the University of Edinburgh site, please contact Ann Wheeler This email address is being protected from spambots. You need JavaScript enabled to view it. for more information.