FCS

Fluorescence correlation spectroscopy is a technique which provides correlation analysis of the fluctuations of fluorescent intensities from single fluorescent molecules. The spatial and temporal analysis obtained is high resolution and can describe mobility coefficients, local concentrations and characteristic rate constants of intermolecular reactions for fluorescent molecules.

FCS is performed on a Leica SP5 SMD Confocal microscope (see specification) equiped with single photon avalanche detectors (SPADs) and integrated Symphotime analysis software.

This microscope is available at the Heriot-Watt University site, contact This email address is being protected from spambots. You need JavaScript enabled to view it..

spt-PALM

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Single particle tracking-photoactivatable localisation microscope (spt-PALM) describes the application o

f PALM to live cells, using photoactivatable fluorophores, typically at the plasma membrane. The high degree of noise detected when imaging single molecules at fast frame rates means de-noising algorithms are required, which can identify each molecule and deliver quantitative data describing each molecule's trajectory. The image on the right shows published data of single SNARE molecules tracked inside a living neuron and the trajectories of those molecules quantified.

spt-PALM is currently available on the epifluorescent Olympus IX81 fully motorised microscope (see specification) controlled by Olympus Cell Xcellence software under TIRFM.

This microscope is available at the Heriot-Watt University site, contact This email address is being protected from spambots. You need JavaScript enabled to view it..