ESRIC recently wrote a review on selecting the best super resolution technique for your research question. The full review can be found here, but the flow diagram below presents a simplified way of choosing the correct technique for your needs.

If you would like further advice, you can email This email address is being protected from spambots. You need JavaScript enabled to view it. or This email address is being protected from spambots. You need JavaScript enabled to view it. to discuss.

Valli J, Garcia-Burgos A, Rooney  LM, Vale de Melo e Oliveira B, Duncan RR & Rickman C (2021) Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique. Journal of Biological Chemistry,  297 (1): 100791, doi: 10.1016/j.jbc.2021.100791